Nanotoxicology, 2013; Early Online, 1–10
© 2013 Informa UK, Ltd.
ISSN: 1743-5390 print / 1743-5404 online
Chiraz Ladhar 1 , Benjamin Geffroy 1 , Sébastien Cambier 1 , Mona Treguer-Delapierre 2 , Etienne Durand 2 , Daniel Brèthes 3 , & Jean-Paul Bourdineaud 1
1 University of Bordeaux, CNRS, UMR 5805, Arcachon Marine Station, Place du Dr Peyneau, 33120 Arcachon, France
2 CNRS, UPR 9048, Université de Bordeaux, Institut de Chimie de la Matière Condensée de Bordeaux, 87 avenue du Dr. Albert
Schweitzer, 33608 Pessac, Cedex, France and 3 CNRS, UMR 5095, Institut de Biochimie et Génétique Cellulaires, Université Victor
Segalen-Bordeaux 2, Bordeaux, France
To address the impact of cadmium sulphide nanoparticles (CdSNPs) of two different sizes (8 and 50 nm), Danio rerio zebrafish were dietary exposed to very low doses: 100 or 40 ng CdSNPs/day/g body weight for 36 or 60 days, respectively. The results obtained using RAPD-PCR genotoxicity test showed genomic alteration since the number of hybridisation sites of the RAPD probes was significantly modified after CdSNPs exposure. In addition, selected stress response genes were either repressed or upregulated in tissues of CdSNPs-exposed fish. Mitochondrial dysfunction was also caused by the presence of CdSNPs in food. Cadmium accumulation in fish tissues (brain and muscles) could only be observed after 60 days of exposure. CdSNPs toxicity was dependent on their size and concentration.
Keywords: cadmium, nanoparticles, zebrafish, genotoxicity, mitochondrial respiration