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Impact of gold nanoparticles on zebrafish exposed to a spiked sediment

Thursday 20 February 2014

http://informahealthcare.com/nan
ISSN: 1743-5390 (print), 1743-5404 (electronic)
Nanotoxicology, Early Online: 1–10
2014 Informa UK Ltd. DOI: 10.3109/17435390.2014.889238

 

Impact of gold nanoparticles on zebrafish exposed to a spiked sediment


Amina Dedeh 1 , Aure ́lie Ciutat 1 , Mona Treguer-Delapierre 2 , and Jean-Paul Bourdineaud 1


1 CNRS, UMR EPOC 5805, University of Bordeaux, Arcachon, France and

2 Institute of Chemistry of Condensed Matter of Bordeaux, University of Bordeaux, CNRS, UPR 9048, Pessac, France

 

Abstract
Increasing use of metallic nanomaterials is likely to result in release of these particles into aquatic environments; nevertheless it is unclear whether these materials present a hazard to aquatic organisms. The impact of contaminated sediment containing 14-nm gold nanoparticles (AuNPs) was investigated in the zebrafish Danio rerio exposed for 20 days to two concentrations, 16 and 55 mg/g dry weight. AuNPs were released from the sediment to the water column, and during this period the mean concentrations of AuNP in the filtered water fraction were 0.25 ± 0.05 and 0.8 ± 0.1 mg/L, respectively. A similar experiment with ionic gold contamination was simultaneously performed to obtain a positive control. AuNP exposure
triggered various effects in fish tissues including modifications of genome composition, shown using a random amplified polymorphic DNA-PCR genotoxicity test. Expression of genes involved in oxidative stress, mitochondrial metabolism, detoxification and DNA repair were also modulated in response to AuNP contamination. Gold altered neurotransmission, since brain acetylcholine esterase activity increased for both tested doses of AuNP but not for ionic gold. Gold accumulation in fish tissues demonstrated the lower bioavailability of AuNP compared to ionic Au, and underlined the higher toxic potential of the nanoparticle form.

 

Keywords : Bioaccumulation, gene expression, genotoxicity, gold nanoparticles, neurotransmission, zebrafish